New from Oxford Expression Technologies
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NEW Products from Oxford Expression Technologies
Go to Oxford Expression Technologies website
The Oxford Expression Technologies family of products has been expanded with new products and more convenient packaging options.

Further enhance protein expression with the new superSf9 cells.

The baculoFECTIN is a new transfection reagent specifically optimised for transfection of insect cells.

Four new pOET Transfer Vectors are now available which code for the addition of C-Terminal and N-Terminal 6xHis-Tag® fusion sequences.

NEW serum and protein free baculoGROW insect cell medium.

Added to the flashBAC product range are the new "baculoCOMPLETE", "flashBAC Selection Box" and "flashBAC 96 Reaction" kits.
Go to Prices on New Products Prices on New Products
NEW Products
Go to superSf9 Cells NEW superSf9 Cells
Go to NEW pOET Transfer Vectors Four NEW Transfer Vectors for C-Terminal and N-Terminal 6xHis-Tag® Fusion Sequences
Go to NEW baculoFECTIN Transfection Reagent NEW baculoFECTIN Transfection Reagent
Go to NEW baculoGROW Serum-free Insect Medium NEW baculoGROW Serum-free Insect Medium
Go to NEW baculoCOMPLETE - ALL-IN-ONE Baculovirus Expression Kit NEW baculoCOMPLETE - ALL-IN-ONE Baculovirus Expression Kit
Go to flashBAC Selection Box NEW flashBAC™ Selection Box
Go to 96 Reaction flashBAC Kits NEW 96 Reaction flashBAC™ Kits
Specific Product/Application Areas:
Go to flashBAC Product Information flashBAC Baculovirus Protein Expression
Go to baculoQUANT Product Information baculoQUANT Virus Titration
Go to titrePLUS Product Information titrePLUS Virus Titration and Protein Expression
Go to pOET Transfer Plasmids Product Information pOET Transfer Vectors
Go to baculoFECTIN Transfection Reagent Information baculoFECTIN Transfection Reagent
Literature
Go to Oxford Expression Technologies Technical Literature Download Oxford Expression Technologies Technical Literature
Go back to Oxford Expression Technologies Products and Applications Oxford Expression Technologies Products and Applications
NEW Transgenic Sf9 Cells
superSf9 Cells
Go to Oxford Expression Technologies websiteThe superSf9 cells are transgenic insect Sf9 cells that have been engineered to stably express an additional protein. The presence of this protein leads to prolonged longevity and increased recombinant protein production in baculovirus infected superSf9 cells compared to standard Sf9 cells. The key features to note about these cell lines are:
  • Modified insect Sf9 cells stably expressing a stabilising protein
  • Prolonged longevity of cells after infection with baculovirus
  • Up to 15-fold increase in protein yield as compared to standard Sf9 cells
 
Each superSf9 cell line has slightly different characteristics to help to enhance the expression of different types of recombinant proteins:
  • superSf9-1 cell line offers a much prolonged expression time that greatly enhances the yield of stable proteins. These cells are suitable for intracellular, membrane and secreted proteins, provided that these proteins are not subject to degradation.
  • superSf9-2 cell line features an intense peak of recombinant protein production which makes them ideal for increasing the yield of toxic or highly unstable proteins.
  • superSf9-3 cell line has a phenotype intermediate between the superSf9-1 and superSf9-2 cells and is a good choice for proteins of mixed or unknown character.
Four NEW Transfer Vectors
Transfer Vectors which code for the addition of C-Terminal and N-Terminal 6xHis-Tag® fusion sequences
Go to Oxford Expression Technologies websiteOxford Expression Technologies has announced the release of four new transfer vectors: pOET1C_6xHis, pOET2C_6xHis, pOET1N_6xHis and pOET2N/C_6xHis. In addition to the choice of either adding a C-Terminal or N-Terminal 6xHis-Tag® fusion sequences, these four new vetors share the same characteristics as the other pOET vectors.
 
  • Add a C-Terminal or N-Terminal 6xHis-Tag® fusion sequence to your expressed protein
  • Small size for cloning - simplifies the cloning process
  • High level expression from the polh promoter
  • Col E1 origin of replication and an ampicillin resistance gene for selection in E. coli
  • Multiple cloning sites for insertion of gene of interest
  • Perfect companion product to the flashBAC™ system
Compatibility with Other Baculovirus systems
ALL pOET Transfer Vectors are  compatible with any baculovirus system that utilises homologous recombination in insect cells including the flashBAC™ products from Oxford Expression Technologies (OET):
Go to pOET Transfer Vectors For further information on the pOET Range of Transfer Vectors
NEW Transfection Reagent
baculoFECTIN Transfection Reagent
Go to Oxford Expression Technologies websiteOxford Expression Technologies has announced the release of baculoFECTIN, a new transfection reagent especially for insect cell lines inlcudings Sf9, Sf21 and T.ni.
Go to baculoFECTIN Transfection Reagent Information For further information on the baculoFECTIN Transfection Reagent
Go to baculoFECTIN Transfection Reagent Information FREE baculoFECTIN Samples
NEW Serum Free Insect Cell Medium
baculoGROW Serum-free Insect Medium
Go to Oxford Expression Technologies websitebaculoGROW is a serum- and protein-free medium. The medium has been specially formulated to facilitate the growth of Spodoptera frugiperda cell lines (Such as Sf9 and Sf21), Drosophila S2 cells and other cells such as Trichoplusia ni (T.ni or Hi-5) cells. baculoGROW can be used for culturing adherent cells and suspension cultures, such as those used in bioreactors. Available in 500mL bottles.
NEW Format flashBAC™ Kits
baculoCOMPLETE - ALL-IN-ONE Baculovirus Expression Kit
Go to Oxford Expression Technologies websiteEspecially formulated for the convenience of scientists who want to try flashBAC and baculovirus protein expression. This ALL-IN-ONE kit contains all necessary components for the expression of recombinant proteins in insect cells. Kit contains flashBACULTRA baculovirus, baculoFECTIN transfection reagent, control transfer plasmid, baculoGROW insect cell media and sf9 insect cells.
flashBAC™ Selection Box
Go to Oxford Expression Technologies websiteOxford Expression Technologies are constantly innovating to enhance the flashBAC baculovirus expression system still further. Now, for the first time, a mixed kit is available that incorporates all four types of flashBAC vector in one package. This allows researchers to easily test each version to see which is perfect for the expression of their protein(s) of interest, and adds additional convenience for laboratories which frequently use different types of flashBAC vector. The kit includes sufficient material for a total of 12 reactions: 3 reaction each of flashBAC, flashBACGOLD and flashBACULTRA and is a great way to help researchers get the very best from the flashBAC range.
NEW 96 Reaction flashBAC™ Kits
Go to Oxford Expression Technologies websiteThe flashBAC system is unique among baculovirus expression systems in that it is compatible with automated systems. The simple one step protocol allows the automation of the generation of multiple recombinant viruses with a robotic liquid handler. This breakthrough allows multiple viruses to be quickly and effortlessly made and screened for protein production. To harness this capability, Oxford Expression Technologies has launched a new 96 reaction size kit of their increasingly popular flashBAC range of kits. In addition to being ideal for automated applications, these kits are also useful for laboratories which frequently do baculovirus protein expression work as the low price per reaction represents excellent value for money. The 96 reaction format allows the very best quality and yield of protein available from a baculovirus expression system to be obtained even more quickly and more conveniently than ever before.
Go to flashBAC Product Information For further informaiton on the range of flashBAC Baculovirus Protein Expression products
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Prices on NEW Products from Oxford Expression Technologies
Item Code1 Product Description Pack Size List Price2
OE-600102 superSf9-1 Cells, 1 x 10E7 cells/mL, 1mL Each P.O.A.
OE-600103 superSf9-2 Cells, 1 x 10E7 cells/mL, 1mL Each P.O.A.
OE-600104 superSf9-3 Cells, 1 x 10E7 cells/mL, 1mL  Each P.O.A.
OE-2001012 pOET1C_6xHis Transfer Vector DNA, 10µg Each $490.00
OE-2001032 pOET2C_6xHis Transfer Vector DNA, 10µg Each $490.00
OE-2001011 pOET1N_6xHis Transfer Vector DNA, 10µg Each $490.00
OE-2001031 pOET2N/C_6xHis Transfer Vector DNA, 10µg Each $490.00
OE-300101 baculoFECTIN Transfection Reagent, 150μL Each $300.00
OE-300102 baculoFECTIN Transfection Reagent, 1mL Each $910.00
OE-500100 baculoGROW Insect Cell Medium, 500mL Each $280.00
OE-400100 baculoCOMPLETE, (5 reactions) Kit P.O.A
OE-100400 flashBAC Selection Box, (9 reactions, 3 of each version of flashBAC) Kit $1,630.00
OE-100152 flashBAC, (96 reactions) Kit $5,900.00
OE-100203 flashBACGOLD, (96 reactions) Kit $6,990.00
OE-100302 flashBACULTRA, (96 reactions) Kit $8,310.00
Note 1: Click on the item code to go to Online Prices and Ordering
Note 2: Prices are exclusive of GST and local packing, handling and delivery charges
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