Oxford Expression Technologies - baculoFECTIN
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Information on the Oxford Expression Technologies baculoFECTIN Transfection Reagent
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baculoFECTIN is a new transfection reagent that has been specifically developed for use in insect cells. It is ideal for use with baculoviruses and is effective with all insect cell lines including Sf9, Sf21 and T.ni. baculoFECTIN is:
 
• RELIABLE - Tried and tested and is regularly used in-house in the Oxford Expression Technologies laboratories
• EFFICIENT - Tests have shown reproducibly higher levels of transfection in insect cells to facilitate high level protein expression
• SIMPLE - Quick and easy protocol: from co-transfection to high titre seed stock
• CELL FRIENDLY - Completely non-toxic so cell health and viability are maintained
• INNOVATIVE - Nanoparticle/polymer duplex optimses the entry into cells and stabilises the DNA with the transfected insect cells
Literature Description Size File
baculoFECTIN Transfection Reagent Protocol
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Specific Product/Application Areas:
Go to flashBAC Product Information flashBAC Baculovirus Protein Expression
Go to baculoQUANT Product Information baculoQUANT Virus Titration
Go to titrePLUS Product Information titrePLUS Virus Titration and Protein Expression
Go to pOET Transfer Plasmids Product Information pOET Transfer Vectors
Go to NEW Products NEW Products
Go to NEW superSf9 Cells NEW superSf9 Cells
Go to NEW Transfer Vectors Four NEW Transfer Vectors for C-Terminal and N-Terminal 6xHis-Tag® Fusion Sequences
Go to baculoFECTIN Transfection Reagent NEW baculoFECTIN Transfection Reagent
Go to NEW baculoGROW Serum-free Insect Medium NEW baculoGROW Serum-free Insect Medium
Go to NEW baculoCOMPLETE - ALL-IN-ONE Baculovirus Expression Kit NEW baculoCOMPLETE - ALL-IN-ONE Baculovirus Expression Kit
Go to NEW flashBAC™ Selection Box NEW flashBAC™ Selection Box
Go to NEW 96 Reaction flashBAC™ Kits NEW 96 Reaction flashBAC™ Kits
Literature
Go to baculQUANT Technical Literature Download Oxford Expression Technologies Technical Literature
Go to Oxford Expression Technologies Products and Applications Oxford Expression Technologies Products and Applications
Special baculoFECTIN Introductory Offer
FREE Samples of baculoFECTIN
For a limited time only, you can request a FREE sample (see Note 1) of baculoFECTIN. Each sample (30µL volume) is sufficient for 5 transfections.

Request your free baculoFECTIN sample by either emailing us at This e-mail address is being protected from spambots. You need JavaScript enabled to view it us or by contacting us via our Technical Enquiry form.

Note 1: Limited to one sample per customer and available only whilst stocks last.
Product Technical Overview
baculoFECTIN
Go to Oxford Expression Technologies website
baculoFECTIN is a new non-toxic transfection reagent that has been optimised for use in insect cells to produce greater levels of transfection - leading to a high level of protein expression.

Although optimised for insect cell lines such as Sf9, Sf21 and T.ni, baculoFECTIN will work will other cell lines as well as primary cell lines and also with a variety of mammalian cell lines. baculoFECTIN consists of two components: a positively charged polymer with DNA binding capacity that is embedded in the second component, a novel porous nanoparticle. The complete nanoparticle complex protects the bound DNA from degradation by nucleases. Only the DNA-nanopartical complex with its unique size can be preferentially taken up by the cells. Within the cells the released DNA-nanoparticle complexes are protected against DNA degradation.

The main benifit of baculoFECTIN compared with lipid based products is that it has a quick, effective and easy protocol from co-transfection to high titre seed stock. It is non-toxic hence cell health and viability are maintained and the nanoparticle/polymer duplex optimises entry into the cells and stabilises the DNA within the cells.

baculoFECTIN is stable for up to one year, is shipped at room temperature and stored at 4°C and is the perfect companion to the flashBAC baculovirus protein expression range of products. It is ideal for use in high-throughput transfection experiments.
 
Improved transfection efficiency with baculoFECTIN:
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